Fluorescence Immunoassay Analyzer

A: Basic principle: a new immunoassay technology using fluorescein-labeled antibodies or antigens as tracers, the principle is similar to ELISA. This method can not only quantify antigens and antibodies in liquids, but also qualitatively and quantitatively identify antigens and antibodies in tissue sections.

A: The normal concentration of cTnI is 1–2 ng/mL or less; after the onset of MI, the concentration of cTnI increases to about 50 ng/mL within 3–6 h reaching as high as 500 ng/mL.

A: In a classical FIA, fluorescent dyes that illuminate in UV light are used to detect the specific binding of an antigen and antibody. The fluorescent dyes, fluorescein isothiocyanate and lissamine rhodamine emit blue-green and orange-red fluorescence, respectively under UV rays in the fluorescence microscope.

A: Immunoassay testing works by using antibodies to detect small biological substances in the blood and other bodily fluids. The method follows the aspect that particular antigens binds to specific newly introduced antibodies, thereby stimulating an immune response.

A: Absorbance detection is based on the absorption that occurs when a compound irradiated with light transitions from the ground state to the excited state. Fluorescence detection is a detection method that uses fluorescence emitted when a compound returns to the ground state after transitioning to the excited state.

A: The analyzer is designed to provide quantitative or qualitative test results by the examination of human samples with specific in vitro diagnostic test units. It offers advantages of high accuracy, strong stability and fast results.

A: Fluoroimmunoassay is a technique that utilizes fluorescent molecules as labels to measure antigen concentrations, with the ability to overcome background fluorescence using time-resolved immunoassay methods.

A: Fluorescence is mainly induced by laser or light-emitting diodes (LEDs). In fluorescence intensity assays, the changes of total light output are monitored and used to measure and quantify a biochemical reaction or binding event. These assays are used to measure enzymatic assays (e.g., proteases, lipases).

A: Most of these immunoassays only give qualitative results, but some also give quantitative results, however in a concentration range that is far too low for TDM purposes as these tests are designed to quantify in the μg/L range (which is necessary to detect antibiotic residues in these dietary products) whilst.

A: 60 min.
Block specimen in Blocking Buffer for 60 min. While blocking, prepare primary antibody in Antibody Dilution Buffer (see product website for recommended dilution range). Aspirate blocking solution then apply diluted primary antibody. Incubate overnight at 4°C.

A: Researchers consider ELISA to be the gold standard of immunoassays. Tests that use ELISA can help diagnose a wide range of conditions, from bacterial and viral infections (like Lyme disease and HIV) to endocrine conditions, like thyroid disease.

A: While immunoassays are highly specific, they may not always be as sensitive as molecular assays. This means that they may not detect infections during the early stages when the patient's immune response is still weak.

A: Blocking buffer should contain heat-inactivated normal serum from the same species as the host of the secondary antibody. Other, less preferred, blocking agents include fetal calf serum (FCS), bovine serum albumin (BSA, Catalog # 5217), casein protein, non-fat dry milk, and gelatin.

A: Block with 5 % normal goat serum/PBS or 1 % BSA/PBS for 45 minutes (no washing required). Dilute the primary antibody in blocking solution and apply it for 2 h (or overnight at 4 °C).

A: Fluorescence immunoassay is a sensitive technique that can be used in the measurement of many compounds, including drugs, hormones, and proteins; in the identification of antibodies; and in the quantification of antigens such as viral particles and, potentially, bacteria.

A: Immunofluorescence can be used on tissues or cell sections to determine presence of different biological molecules which also includes proteins, carbohydrates, etc. Also used in molecular biology for visualization of cytoskeletons such as intermediate filaments.

A: The fluorescent dyes, fluorescein isothiocyanate and lissamine rhodamine emit blue-green and orange-red fluorescence, respectively under UV rays in the fluorescence microscope. The advantages of an FIA method include higher sensitivity detection of the analyte, simplified reagents, and simpler assay designs.

A: In clinical immunodermatology, there are three basic types of immunofluorescence techniques: direct immunofluorescence (DIF), indirect immunofluorescence (IIF) [Figure - 1], and complement binding indirect immunofluorescence.

A: The Fluorescence Immunoassay Analyzer platform is a rapid, quantitative instrument that tests quantitative items such as cardiac markers inflammation, hormone, diabetes, etc.

A: Fluorescence spectroscopy is used in, among others, biochemical, medical, and chemical research fields for analyzing organic compounds. There has also been a report of its use in differentiating malignant skin tumors from benign.