HBsAb Rapid Test

• Easy to use
• No equipment required
• High sensitivity and specificity
• Fast results at 15 minutes

Viral hepatitis is a systemic disease primarily involving the liver. Most cases of acute viral hepatitis are caused by Hepatitis A virus, Hepatitis B virus (HBV) or Hepatitis C virus. The complex antigen found on the surface of HBV is called HBsAg. The presence of HBsAg in serum or plasma is an indication of an active Hepatitis B infection, either acute or chronic. The antibody to HBsAg, HBsAb, may not become detectable for 3-6 months after acute infection. It is associated with resolution of the illness. This antibody is recognized as the marker of immunity to HBV. As a result, vaccination against HBV was introduced to control the morbidity and mortality associated with the virus. As part of the World Health Organization
(W.H.O) program for the control of Hepatitis B, many people, especially new born infants, receive vaccination. The minimum standard titer of HBsAb is 10 mIU/mL for protective immunity to HBV.¹ Unfortunately, approximately 5-15% of healthy immunocompetentindividuals either does not exhibit an antibody response to the existing recombinant vaccination or respond poorly.²The HBsAb Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid test to qualitatively detect the presence of HBsAb in serum or plasma specimen. The test utilizes a double antigen sandwich system to detect as low as 10mIU/ml of HBsAb in serum or plasma.

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.
1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it within one hour.
2. Place the cassette on a clean and level surface.
For Serum or Plasma specimen:
 Hold the dropper vertically and transfer 3 drops of serum or plasma (approximately 75 µL) to the specimen well of test Cassette and start the timer. See illustration below.
For Venipuncture Whole Blood specimen:
 Hold the dropper vertically and transfer 2 drops of whole blood (approximately 50 µL) to the specimen area, then add 1 drop of buffer (approximately 40 µL), and start the timer. See illustration below.
For Fingerstick Whole Blood specimen:
 To use a capillary tube: Fill the capillary tube and transfer approximately 50 µL of fingerstick whole blood specimen to the specimen area of test cassette, then add 1 drop of buffer (approximately 40 µL) and start the timer. See illustration below.
3. Wait for the colored line(s) to appear. Read results at 15 ~20 minutes. Do not interpret the result after 20 minutes

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