Cryptosporidium Rapid Test
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Cryptosporidium Rapid Test

The Cryptosporidium Antigen Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of Cryptosporidium Antigen in human feces specimens to aid in the diagnosis of Cryptosporidium Antigen infection.
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Description

Technical Parameters

advantage

 

  • Easy to use
  • No equipment required
  • High sensitivity and specificity
  • Fast results at 10 minutes

 

Summary

 

Cryptosporidiosis is a mucosal infection caused by apicomplexan parasites of the genus Cryptosporidium, which generally develop in the epithelial cells of the small intestine.1 It is usually diagnosed by microscopic detection of Cryptosporidium oocysts in stool specimens. The parasite is protected by an outer shell that allows it to survive outside the body for long periods of time and make it very resistant to chlorine-based disinfectants. The infection is acquired through the ingestion of oocysts, frequently through the consumption of contaminated water or food. 2 It can also spread through coughed fomites of an infected individual.3 Cryptosporidium antigen detec-tion by immunoassays has become a well-established aid to microscopic examination for the diagnosis of cryptosporidiosis. Both the disease and the parasite are commonly known as"Crypto".The Cryptosporidium Antigen Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of Cryptosporidium Antigen in human feces specimen,providing results in 10 minutes. The test utilizes antibody specific for Cryptosporidium Antigen to selectively detect Cryptosporidium Antigen from human feces specimens.

 

Convenient Procedure

 

product-443-398

 


Allow the test, specimen, buffer, and/or controls to reach room temperature (15-30°C) prior to testing.
1. To collect fecal specimens:
Collect sufficient quantity of feces (1-2 mL or 1-2 g) in a clean, dry specimen collection container to obtain enough virus particles. Best results will be obtained if the assay is performed within 6 hours after collection. Specimen collected may be stored for 3 days at 2-8°C if not tested within 6 hours. For long term storage, specimens should be kept below -20°C.
2. To process fecal specimens:
 For Solid Specimens:
Unscrew the cap of the specimen collection tube,then randomly stab the specimen collection applicator into the fecal specimen in at least 3 different sites to collectapproximately 50 mg of feces (equivalent to 1/4 of a pea). Do not scoop the fecal specimen.
 For Liquid Specimens:
Hold the dropper vertically, aspirate fecal specimens, and then transfer 2 drops (approximately 50 µL) into the specimen collection tube containing the extraction buffer.Tighten the cap onto the specimen collection tube, then shake the specimen collection tube vigorously to mix the specimen and the extraction buffer.
3. Bring the pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it within one hour. Best results will be obtained if the test is performed immediately after opening the foil pouch.
4. Hold the specimen collection tube upright and break off the tip of the specimen collection tube. Invert the specimen collection tube and transfer 2 full drops of the extracted specimen (approximately 80 µL) to the specimen well (S) of the test cassette, then start the timer. Avoid
trapping air bubbles in the specimen well (S). See illustration below.
5. Read the results at 10 minutes after dispensing the specimen. Do not read results after 20 minutes.
Note: If the specimen does not migrate (presence of particles), centrifuge the diluted sample contained in the extraction buffer vial. Collect 80 µL of supernatant, dispense into the specimen well (S). Start the timer and continue from step 5 onwards in the above instructions for use.

 

Accurate Results

 

product-651-169

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