FOB Transferrin Rapid Test Cassette

• Easy to use 
• No equipment required
• High sensitivity and specificity
• Fast results at 5 minutes

Many diseases can cause hidden blood in the feces. This is also known as Fecal Occult Blood (FOB), Human occult Blood, or Human Hemoglobin. In the early stages, gastrointestinal problems such as colon cancer, ulcers, polyps, colitis, diverticulitis, and fissures may not show any visible symptoms, only occult blood. Fecal occult blood testing can provide clues as to subtle blood loss in the gastrointestinal tract. Positive tests ("positive stool") warrant further investigation for a malignancy such as colorectal cancer (that is one of the leading causes of illness and death in the Western word) or gastric cancer.The presence of human hemoglobin in feces is inadequate as a screening test for stomachcancer (upper gastrointestinal disorders), because of human hemoglobin derived from theupper digestive tract is broken down in the intestinal tract (the antigenicity is lost). Detection offecal Transferrin, which is more resistant to the metabolism in the intestine than that of the Hemoglobin, provides the best complementary way of diagnosing the disease in the upper digestive tract. Transferrin is a blood plasma protein for iron ion delivery. Although iron bound to transferring is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool with the highest rate of turnover (25 mg/24 h).Transferrin imbalance can have serious health effects for those with high serum transferring levels. A patient with an increased serum transferring level suffers from iron deficiency anemia that is one of the most important symptoms when a tumor has caused chronic occult bleeding.The FOB/Transferrin Rapid Test Cassette (Feces) is a rapid test to qualitatively detect low levels of Human Hemoglobin and Transferrin in feces. The test uses a double antibody sandwich assay to selectively detect Human Hemoglobin at 10ng/ml or higher and Transferrin at 4ng/ml or higher. In addition, unlike guaiac assays, the accuracy of the test is not affected by the diet of the patients.

Collect fecal specimens:Collect feces in a clean, dry specimen collection container. Best results will be obtained if the assay is performed within 6 hours after collection. Specimen collected may be stored for 3 days at 2-8℃ if not tested within 6 hours.
Process fecal specimens:Unscrew the cap of the specimen collection tube, then randomly stab the specimen collection applicator into the fecal specimen in at least 3 different sites. Do not scoop the fecal specimen.Screw on and tighten the cap onto the specimen collection tube, then shake the specimen collection tube vigorously to mix the specimen and the extraction buffer. Specimens prepared in the specimen collection tube may be stored for 6 months at -20℃ if not tested within 1 hour after preparation.
Bring the pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it within one hour. Best results will be obtained if the test is performed immediately after opening the foil pouch.
Hold the specimen collection tube upright and open the cap onto the specimen collection tube. Invert the specimen collection tube and transfer 2 full drops of the extracted specimen (approximately 80 µL) separately to the specimen well (S) of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well (S). See illustration below.
Read results at 5 minutes. Do not read results after 10 minutes.

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