Toxoplasma Test Cassette

• Easy to use
• No equipment required
• High sensitivity and specificity
• Fast results at 10 minutes

T. gondii is an obligate intracellular protozoan parasite with a worldwide distribution1,2 .

Serological data indicates that approximately 30% of the population of most industrialized nations is chronically infected with the organism3 . A variety of serologic tests for antibodies to T. gondii have been used as an aid in diagnosis of acute infection and to assess previous exposure to the organism. These tests are the Sabin Feldman dye test, direct agglutination, indirect hemagglutination, latex agglutination, indirect immunofluorescence, and ELISA4-7 . Recently, lateral flow chromatographic immunoassay, such as The Toxo IgG Rapid Test Cassette was introduced into the clinic for the serodiagnosis of T. gondii infection.

1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.

2. Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 1 drop of serum or plasma (Approx.10μl) and 2 drops of buffer (Approx.80μl) to the specimen well of the test cassette. Avoid trapping air bubbles in the specimen well. See the illustration below.

3. Wait for the colored line(s) to appear. The result should be read at 15 minutes. Do not interpret results after 20 minutes.

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