Myoglobin Rapid Test

• Easy to use
• No equipment required
• High sensitivity and specificity
• Fast results at 10 minutes

Myoglobin (MYO) is a heme-protein normally found in skeletal and cardiac muscle with a molecular weight of 17.8kDa. It constitutes about 2 percent of total muscle protein and is responsible with transporting oxygen within the muscle cells.1 When the muscle cells are damaged, Myoglobin is released to the blood rapidly due to its relatively small size. Following the death of tissue associated with MI, Myoglobin is one of the first markers to rise above normal levels. The level of Myoglobin increases measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours and returning to baseline within 24-36 hours.2,3 A number of reports suggest the measurement of Myoglobin as a diagnostic aid in confirming the absence of myocardial infarction with negative predictive values of up to 100% reported at certain time periods after onset of symptoms.4

The Myoglobin Rapid Test Cassette (Whole Blood/Serum/Plasma) is a simple test that utilizes a combination of anti-Myoglobin antibody coated particles and capture reagents to qualitatively detect Myoglobin in whole blood, serum or plasma. The minimum detection level is 50ng/mL.

1. Allow the pouch to equilibrate to ambient temperature before opening. Retrieve the test cassette from the sealed pouch and use it within sixty minutes.

2. Position the cassette on a flat and hygienic surface.

For Serum or Plasma samples:

- Hold the dropper upright and dispense 1 drop of serum or plasma (around 25μL) into the sample well of the test cassette, then add 1 drop of buffer (around 40μL) and activate the timer. Refer to the illustration provided.

For Venipuncture Whole Blood samples:

- Hold the dropper upright and dispense 2 drops of whole blood (around 50μL) onto the sample area, then add 1 drop of buffer (around 40μL), and activate the timer. Refer to the illustration provided.

For Fingerstick Whole Blood samples:

- To use a capillary tube: Fill the capillary tube and transfer approximately 50μL of fingerstick whole blood sample to the sample area of the test cassette, then add 1 drop of buffer (around 40μL) and activate the timer. Refer to the illustration provided.

3. Observe for the appearance of the colored line(s). Interpret the results at the 5-minute mark. Do not interpret the results after 20 minutes.

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