NT-proBNP Rapid Test

• Easy to use
• No equipment required
• High sensitivity and specificity
• Fast results at 10 minutes

Congestive Heart Failure (CHF) is described as a condition in which the heart is unable to maintain adequate circulation of blood in the tissues of the body or to pump out the venous blood returned to it by the venous circulation CHF affects nearly 17 million people worldwide.

NT-proBNP, N-terminal fragment of proBNP, is cleaved from the precursor peptide proBNP. It shows close correlation with the severity of heart failure. NT-proBNP is widely recognized as a definitive marker for the diagnosis of CHF. Some amount of NT-proBNP is available in the serum.

However, elevated levels of NT-proBNP indicate the presence of CHF. The high sensitivity of NT-proBNP allows also the detection of mild forms of cardiac dysfunction in asymptomatic patients with structural heart disease.1, 2, 3 The European Society of Cardiology Task Force for the Diagnosis and Treatment of Chronic Heart Failure recommend in their guidelines that natriuretic peptides including NT-proBNP may be most useful clinically as a rule out test due to consistent and very high negative predictive values.4

The NT-proBNP Rapid Test Cassette (Whole Blood/Serum/Plasma) is a simple test that utilizes a combination of anti-NT-proBNP antibodies coated particles and capture reagents to detect NT-proBNP in whole blood, serum or plasma. The minimum detection level is 450 pg/mL.

1. Bring the pouch to room temperature before opening it. Remove the test from the sealed pouch and use it within one hour. Best results will be obtained if the test is performed immediately after opening the foil pouch.

2. Place the test on a clean and level surface.

For Serum or Plasma specimens: Hold the dropper vertically and transfer 2 drops of serum or plasma (approximately 50 μL) and 1 drop of buffer (approximately 40ul) to the specimen well (S) of the test , then start the timer. See illustration below.

For Venipuncture Whole Blood specimens: Hold the dropper vertically and transfer 3 drops of venipuncture whole blood (approximately 75μL) and 1 drop of buffer (approximately40μL) to the specimen well (S) of the test , then start the timer. See illustration below.

For Fingerstick Whole Blood specimens: To use a capillary tube: Fill the capillary tube and transfer approximately 75 μL of fingerstick whole blood specimen to the specimen area of test cassette, then add 1 drop of buffer (approximately 40 μL) and start the timer. See illustration below.

3. Wait for the colored line(s) to appear. Read results at 10minutes. Do not interpret results after 20minutes.

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