Chikungunya IgM Rapid Test Cassette

• Easy to use
• No equipment required
• High sensitivity and specificity
• Fast results at 1 minutes

Chikungunya is a rare viral infection transmitted by the bite of an infected Aedes aegyptimosquito. It is characterized by a rash, fever and severe joint pain (arthralgias) that usually lasts for three to seven days. The name is derived from the Makonde and meaning"that which bends up" in reference to the stooped posture developed as a result of the arthritic symptoms of the disease. It occurs during the rainy season in tropical areas of the world, primarily in Africa, South-East Asia, southern India and Pakistan.The symptoms are most often clinically indistinguishable form those observed in dengue fever. Unlike dengue, hemorrhagic manifestations are relatively rare and most often the disease is a self-limiting, febrile illness. Dual infection of dengue and chikungunya is also possible as has been reported in India. Therefore, it is very important to clinically distinguish dengue from CHIK infection.CHIK is diagnosed based on serological analysis and viral isolation in mice or tissue culture. An IgM immunoassay is the most practical lab test method.The Chikungunya IgM Rapid Test utilizes recombinant antigens derived from its structure protein; it detects IgM anti-CHIK in patient serum or plasma within 15 minutes. The test can be Performed by untrained or minimally skilled personnel without cumbersome laboratory equipment.

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.
1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible.
2. Place the cassette on a clean and level surface.
 For Serum or Plasma specimen:
Hold the dropper vertically and transfer 1 drop serum or plasma (approximately 40 µL) to the specimen area, then add 1 drop of buffer (approximately 40 µL),and start the timer. See illustration below.
 For Venipuncture Whole Blood specimen:
Hold the dropper vertically and transfer 1 drop whole blood (approximately 40 µL) to the specimen area, then add 1 drop of buffer (approximately 40 µL), and start the timer. See illustration below.
 For Fingerstick Whole Blood specimen:
To use a capillary tube: Fill the capillary tube and transfer approximately 40 µL of fingerstick whole blood specimen to the specimen area of test cassette, then add 1drop of buffer (approximately 40 µL) and start the timer. See illustration below.
3. Wait for the colored line(s) to appear. Read results at 15 minutes. Positive results can be visible in as soon as 1 minute. Do not interpret the result after 15 minutes

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