Typhoid Rapid Test
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Typhoid Rapid Test

The Typhoid Rapid Test Cassette is a rapid chromatographic immunoassay for the simultaneous detection and differentiation of IgG and IgM types of antibodies against Salmonella typhi (S. typhi) in human serum or plasma. It is intended to be used as a screening test as an aid in the diagnosis of infection with S. typhi. Any reactive specimen with the Typhoid rapid test cassette needs to be confirmed with alternative testing method.

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Description

Technical Parameters

advantage

Easy to use
No equipment required
High sensitivity and specificity
Fast results at 10 minutes

Summary

Typhoid fever is caused by S. typhi, a Gram-negative bacterium. World-wide an estimated 17 million cases and 600,000 associated deaths occur annually1 . Patients who are infected with HIV are at significantly increased risk of clinical infection with S. typhi2 . Evidence of h. pylori infection also presents an increased risk of acquiring typhoid fever. 1-5% of patients become chronic carrier harboring S. typhi in the gallbladder.

The clinical diagnosis of typhoid fever depends on the isolation of S. typhi from blood, bone marrow or a specific anatomic lesion in the facilities that cannot afford to perform this complicated and time consuming procedure, WidalTest (also referred as Weil-Felix Test) is used to facilitate the diagnosis. However, many limitations lead to difficulties in the interpretation of the Widal test 3, 4 .

In contrast, the Typhoid Rapid Test Cassette is a simple and rapid laboratory test. The test simultaneously detects and differentiates the IgG and the IgM antibodies to S. typhi specific antigen5 in serum or plasma thus aid in the determination of current or previous exposure the S. typhi.

Convenient Procedure

1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.

2. Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 1 drop of serum or plasma (40 ml) to the specimen well of the test cassette, then add 1 drop of buffer (40 ml) and start the timer. Avoid trapping air bubbles in the specimen well. See the illustration below.

3. Wait for the colored line(s) to appear. The result should be read at 15 minutes. Do not interpret results after 15minutes

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