Semi-quantitative Micro-Albumin Rapid Test
The micro-albumin test is an immune-chemical lateral flow test. It serves the semi-quantitative detection of small quantities of albumin in urine. Within a concentration range of 20 mg/L –100 mg/L the color intensity of the test result line decreases continuously, so that the albumin quantity can be read off on the basis of a color scale. Albumin concentrations above these values are indicated by the complete disappearance of the test result line.The micro-albumin test is exclusively intended for professional use and is visually evaluated. The product serves as a first screening of urine samples and provides a provisional analytic result, since fluctuations in the urine concentration are not considered. Positive test results above 20 mg/L should therefore be confirmed by a more specific quantitative method. Clinical consideration should be applied to the interpretation of each micro albumin test, particularly if a preliminary positive test result is present.
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Description
Technical Parameters
advantage
- Easy to use
- No equipment required
- High sensitivity and specificity
- Fast results at 5 minutes
Summary
The micro-albumin test is a competitive immunoassay, in which immobilized human albumin of the test competes with the albumin which may be present in the urine for a limited number of antibody binding sites. The test strip is in the test line region (T-region) coated with human albumin.
Convenient Procedure
After opening the tube the required amount of tests should be carried out immediately. Tests, which have been stored refrigerated, should be equilibrated to room temperature before opening to avoid condensation of humidity on the test.
1. Open the tube and remove the test strip at the handle. Mark the strip if necessary for identification purposes. Avoid direct contact of the white membrane in the center of the test strip. Close the tube immediately afterwards.
2. Dip the other end of the test strip for at least 10 seconds into the urine sample. Make sure that the test is not submerged deeper than the MAX mark. The urine may not have direct contact with the white membrane, since this makes the test useless. To make sure the absorbed amount of liquid is sufficient, it is recommended to wait for the release of the color marked antibodies. This can be recognized by the moving of the red liquid front along the membrane, or by the development of the control line.
3. Remove the test strip and place it on a clean surface which does not extract humidity (e.g. on the test pouch). Start the timer.
4. Read the test result after 5 minutes, by comparing the color intensity of the test result line within the T-range with the color scale. The evaluation should take place no later than 10 minutes after the test. Please adhere strictly to this time. Longer or shorter response times affect the color intensity of the test result line and obstruct a safe semi-quantitative evaluation.
1. Open the tube and remove the test strip at the handle. Mark the strip if necessary for identification purposes. Avoid direct contact of the white membrane in the center of the test strip. Close the tube immediately afterwards.
2. Dip the other end of the test strip for at least 10 seconds into the urine sample. Make sure that the test is not submerged deeper than the MAX mark. The urine may not have direct contact with the white membrane, since this makes the test useless. To make sure the absorbed amount of liquid is sufficient, it is recommended to wait for the release of the color marked antibodies. This can be recognized by the moving of the red liquid front along the membrane, or by the development of the control line.
3. Remove the test strip and place it on a clean surface which does not extract humidity (e.g. on the test pouch). Start the timer.
4. Read the test result after 5 minutes, by comparing the color intensity of the test result line within the T-range with the color scale. The evaluation should take place no later than 10 minutes after the test. Please adhere strictly to this time. Longer or shorter response times affect the color intensity of the test result line and obstruct a safe semi-quantitative evaluation.
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